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After eight years of rehabilitation, a profitable launch and 97 days of monitoring Bob within the nice blue ocean, the Two Oceans Aquarium Basis has revealed some thrilling information: Bob is a lady!
Laura du Toit, Two Oceans Aquarium’s content material advertising coordinator, revealed the entire particulars about this thrilling improvement.
In response to the write-up, the Turtle Conservation Centre’s specialised turtle vet, Dr Bernice van Huyssteen, mentioned that it may be difficult to find out a turtle’s intercourse. By taking a blood pattern for a DNA (genetic) check, which is a straightforward and accessible check, it’s attainable to find out the intercourse of the vast majority of animals. Nonetheless, this sort of testing is inadequate for turtles.
‘In sea turtles, the women and men are genetically the identical,’ defined Dr Bernice. ‘Turtles’ sexes are decided by the temperature of the sand during which they hatch: if the eggs incubate at temperatures under 27.7°C, they are going to be male, whereas temperatures above 31°C will end in extra feminine hatchlings. Subsequently, female and male turtles’ genetics are the identical. That is known as temperature-dependent intercourse dedication.’
Different strategies of figuring out the intercourse of juvenile turtles are additionally troublesome. Turtles’ intercourse organs (ovaries in females and testes in males) look very comparable on ultrasound examination earlier than they attain maturity.
So the veterinary workforce at Two Oceans Aquarium confronted the dilemma of figuring out Bob’s intercourse as a comparatively immature inexperienced turtle. Dr Bernice got here throughout a 2015 examine that used testosterone ranges to find out whether or not inexperienced turtles have been male or feminine throughout her analysis. Increased testosterone ranges point out a male turtle, whereas decrease ranges point out a feminine turtle. The examine’s reliability was demonstrated when the outcomes have been confirmed when the turtles within the examine matured.
The veterinary workforce took blood samples from Bob on the day she was launched. This was placed on maintain whereas they carried out intercourse dedication analysis and deliberate the checks. Dr Bernice delivered the frozen blood pattern to Pretoria’s specialist endocrine laboratory, the place the testosterone check was carried out.
Lastly, the Two Oceans Aquarium workforce in contrast Bob’s testosterone ranges to the examine’s regular ranges for women and men. It was decided that Bob is feminine!

Dr Bernice carried out a medical examine and took a blood pattern earlier than Bob’s launch. Image: Two Oceans Aquarium Basis
What does this imply for Bob?
‘Someday, Bob will hopefully return to the seaside the place she hatched to convey forth a brand new technology of turtles!’ mentioned Dr Bernice.
Previous to the check outcomes, the Turtle Conservation Centre workforce assumed there can be little tangible proof of Bob’s future actions; male turtles hardly ever come ashore and spend the vast majority of their lives at sea, whereas females return to the identical seaside each few years to nest.
The Two Oceans Aquarium Basis is happy about the potential for Bob coming ashore to nest now that her intercourse has been confirmed as feminine. They can observe her as a result of she is provided with a satellite tv for pc, flipper and acoustic tags. Feminine inexperienced turtles, like Bob, lay as much as 2 300 eggs of their lifetime and nest each two to a few years.
Talitha Noble, supervisor of the Turtle Conservation Centre and certainly one of Bob’s major carers, was overjoyed with the information.
She mentioned, ‘There’s an opportunity Bob might be noticed on the seaside when she (hopefully) nests someday, and we might proceed our journey together with her. We might even retag her to proceed monitoring her actions! The concept of a lot of little Bobbies on the planet is an thrilling one!’
How does the turtle workforce really feel in regards to the outcomes?
In response to du Toit, Dr Bernice was ecstatic to be taught that Bob was feminine.
‘Previously, we’ve tried to find out her intercourse by way of ultrasound with out success. It was so nice to lastly get a solution! I used to be very happy that the check labored and that we obtained an correct end result,’ mentioned Dr Bernice.
du Toit defined that this check establishes a precedent for future intercourse determinations of inexperienced turtles on the Turtle Conservation Centre.
In response to Dr Bernice, Bob is the primary turtle in South Africa whose intercourse has been efficiently confirmed by way of testosterone testing. It is a enormous accomplishment for the Turtle Conservation Centre, which regularly works with stranded inexperienced turtles.
The turtle workforce will be capable of contribute to the scientific database on inhabitants demographics through the use of testosterone checks on stranded inexperienced turtles. Testosterone testing additionally provides to their understanding of the conservation standing of inexperienced turtles.

Talitha Nobe with Bob throughout her rehabilitation. Image: Two Oceans Aquarium Basis
Du Toit wrote, ‘Bob’s journey highlights the significance of our work on the Turtle Conservation Centre. Bob arrived as an injured little turtle, shortly changing into an anti-plastic ambassador and a beloved member of the aquarium household. Now, she has the potential to mom a brand new technology of inexperienced turtles. This story reveals why rehabilitation is so necessary: after eight years of care, enrichment and remedy, Bob went from being “unreleasable” (a hopeless case) to probably persevering with the inexperienced turtle species within the wild.’
Is Bob’s identify going to vary?
Bob will all the time be Bob to the Two Oceans Aquarium household, based on Du Toit, and so they assume she ought to keep that manner!
Written by Sarah du Toit
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